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. Author manuscript; available in PMC: 2017 Jul 17.
Published in final edited form as: Biochem J. 2016 Jan 8;473(6):743–755. doi: 10.1042/BJ20150586

Figure 2. 2DG/AA increases ceramides, C16-dhCer and sphingoid bases in MEFs.

Figure 2

WT MEFs were treated with vehicle or 2DG (20 mM) and AA (7.5 µM) for 12 or 36 h. Cells were harvested, lipids extracted and subjected to HPLC–MS/MS analysis for quantification. (A) Levels of the individual ceramide species (C14–C24). (B) Levels of C16-dhCer, dhSph, Sph and S1P. Results are normalized to lipid phosphate. Results are the means ± S.D., n = 3.