Skip to main content
. Author manuscript; available in PMC: 2017 Jul 17.
Published in final edited form as: Biochem J. 2016 Jan 8;473(6):743–755. doi: 10.1042/BJ20150586

Figure 3. Cells lacking nCDase are resistant to 2DG/AA-induced necroptotic cell death.

Figure 3

Percentage of LDH release from (A) WT or nCDase−/− MEFs treated with either vehicle (CT, control) or 2DG (20 mM) and AA (7.5 µM) for 24 h. (B) Western blot analysis of lysates from WT and nCDase−/− MEFs treated with vehicle (CT, control) or 2DG (20 mM) and AA (7.5µM) for 24 h. (C) Percentage of LDH release from non-targeting shRNA (pLK0.1) or shRNA-nCDase WT MEFs treated with vehicle (CT) or with 2DG (20 mM) and AA (7.5 µM) for 24 h. (D) WT and nCDase−/− MEFs stably overexpressing human nCDase treated with 2DG (20 mM) and AA (7.5µM) for 24 h. (A) Results are the means ± S.D. for three independent experiments each with technical duplicates. (B) A representative Western blot from an experiment performed on three independent occasions. (C and D) Results are expressed as means ± S.D. for two independent experiments each with technical triplicates. (*P < 0.05 as determined by Student’s t test or one-way ANOVA where appropriate). NS indicates not significant.