Figure 7. 2DG/AA treatment induces increased mitophagy in nCDase^−/− cells.

WT and nCDase^−/− cells were pre-incubated with MitoTracker Red CMXRos (500 nM) at 37°C for 1 h in Opti-MEM to reduce MitoTracker oxidation. Cells were treated with 2DG (20 mM) and AA (7.5 µM) for the indicated times. CT denotes control vehicle-treated cells. Cells were fixed, permeabilized and imaged via confocal microscopy.