Table 3.
Summary of expression results for the four dntms in sole. Results obtained about gene expression patterns as determined by qPCR and WISH in embryos, larvae and juveniles and their regulation by 5-AzaC and temperature
| Expression patternsa | Regulationb | |||||
|---|---|---|---|---|---|---|
| Embryos | Lecitotrophic larvae | WISH patterns in larvae | Juveniles | 5-AzaC | Temp | |
| dnmt1 | -- | ↓↓ | Brain, spinal cord, eyes, pharynx and intestine | Brain | ↓ | --- |
| dnmt3aa | ↑↑ | ↓ | Somites, pronephric duct, brain, spinal cord, eyes, pharynx and intestine | Spleen and gills | ↑ | ↑↑ |
| dnmt3ab | ↑ | ↑ | Brain, eye layers, spinal cord and intestine | Heart and brain | -- | ↑ |
| dnmt3bb.1 | ↓ | ↓ | Brain, pectoral fin buds, pronephric duct, eyes, intestine, pharynx and branchial arches | Gills | -- | --- |
aThe increase (↑) or decrease (↓) of gene expression from gastrula to hatch in embryos or during lecitotrophic larval development is shown
bThe up- (↑) or downregulation (↓) of gene expression after 5-AzaC treatments or in response to low temperature (16 °C) is shown
The arrows indicate if expression is differentially induced (↑ = or reduced (↓) for each factor and their number denote the intensity of the response