Fig. 4.

SUP-46 protects from heat stress and localizes to stress granules. a SUP-46 accumulates in stress granules and is enriched in the nucleus within 10 min of heat stress, and dissipates from stress granules within 25–35 minutes at 20 °C. Inset numbers are duration of heat stress in individual embryos (top row), or recovery in the 10 min heat stressed embryo (middle and bottom rows). Images are single confocal optical sections of embryos that express SUP-46::FLAG::GFP. Scale bars represent 15 μm. b SUP-46 is essential for optimal fecundity, especially at elevated temperature. Data points represent brood counts of individual hermaphrodites. Hatched line and error bars are the mean ± SD (n = 11–35). *Significantly different from wildtype [wt; sup-46(+)] at that temperature; P < 0.05. Inset shows hatchling number expressed as percentage of wildtype, to illustrate the increased requirement for SUP-46 at 26 °C. c sup-46 mutant embryos have a shorter acute heat stress survival time than wildtype (n = 10–14; 20 embryos/replicate). Error bars are SD. Allelic differences in time to lethality in 50% of the population [LT₅₀ (min)] are shown on the right, with shorter heat stress survival indicated by a smaller LT₅₀. P values for comparison of the different alleles are indicated. ns, not significantly different