Fig. 1.

Expression profiles in response to single and combined stresses in Brassica nigra plants. a Correspondence analysis of expression profiles including all induced or repressed genes in at least one experiment (−0.585 < log₂ ratio > 0.585, P < 0.05, n = 961). Clustering and node length calculations were performed with MultiExperiment Viewer 4.8.1 using Pearson’s correlation. b Proportion of P. brassicae-upregulated genes that are also upregulated during a combined stress. Each bar segment (yellow, green, blue) represents a different combined stress. The proportion of genes specifically induced by P. brassicae is shown in orange. The number of genes regulated by herbivory (upregulated, n = 218; downregulated, n = 49) is set to 100%. (c) Proportion of P. brassicae-downregulated genes also downregulated during a combined stress. P.b., P. brassicae larvae; EE, P. brassicae egg extract; Xcr, Xanthomonas campestris pv. raphani; Aphids, Brevicoryne brassicae