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. 2017 Jul 17;17:127. doi: 10.1186/s12870-017-1074-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Expression of JA- and SA-related genes. a Experimental design. b Expression of B. nigra genes was measured by QPCR and normalized to the housekeeping gene BnSAND. Leaf discs (blue circle) were collected on 5-week-old B. nigra plants pretreated for 3 days with P. brassicae egg extract (EE) or Xanthomonas campestris pv. raphani (Xcr) and further challenged with P. brassicae (P) feeding for 24 h at the site of treatment. Untreated plants (CTL) or plants exposed to a single stress were included. Means (± SE) of three technical replicates are shown. These experiments were repeated at least twice with similar results. Different letters indicate significant differences (two-way ANOVA followed by Tukey’s honest significant difference test, P < 0.05)