Fig. 4.

Effects of suppressing EgcrtB on E. gracilis cells. Cells treated with or without EgcrtB-dsRNA were cultured for 7 days at 25 °C under continuous illumination at 55 μmol m⁻² s⁻¹. a Semi-quantitative reverse transcription-PCR analysis of EgcrtB expression in cells with (+) or without (−) EgcrtB-dsRNA. Non-electroporated cells (n) were used as the control. GAPDH expression was used as an internal control for the semi-quantitative RT-PCR analysis. b Cells treated with or without EgcrtB-dsRNA and visual appearance of culture medium (insets) after cultivation for 3 and 7 days. Scale bar, 20 μm. c TEM images of intracellular structures (left column: scale bar, 5 μm) and sections of chloroplasts (right column: scale bar, 500 nm) of cells treated with or without EgcrtB-dsRNA. C, chloroplast; CV, contractile vacuole; LG, lipid globule; N, nucleus; P, paramylon; PG, plastoglobule