Table 2.
Effects of suppressing EgcrtB on cell concentration and chlorophyll content of E. gracilis
| Treatment | Cell concentration | Chlorophyll content (nmol 106 cells−1) | Chlorophyll a/b | ||
|---|---|---|---|---|---|
| Cultured for 3 days (×104 cells ml−1) | Cultured for 7 days (×106 cells ml−1) | a | b | ||
| non-electroporated | 7.7 ± 0.7a | 1.9 ± 0.0a | 7.7 ± 0.9a | 1.1 ± 0.1a | 6.9 ± 0.3a |
| EgcrtB-dsRNA(−) | 5.5 ± 0.7b | 1.8 ± 0.1a | 8.5 ± 1.6a | 1.2 ± 0.2a | 7.3 ± 0.3a |
| EgcrtB-dsRNA(+) | 3.3 ± 0.2c | 0.3 ± 0.0b | 1.3 ± 0.1b | 0.2 ± 0.0b | 6.1 ± 0.5b |
Data represent mean ± SD of biological replicates. The number of biological replicates was as follows: cell concentration, n = 3; chlorophyll content and chlorophyll a/b ratio, n = 4. Different letters in each column indicate a significant differences (P < 0.05, Tukey’s test)