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. 2017 Jul 17;12(7):e0180861. doi: 10.1371/journal.pone.0180861

Fig 7. Expression driven by a predicted enhancer.

Fig 7

A 900bp sequence, located between 3130 and 2230 bp upstream of the eve TSS, was placed upstream of a lacZ reporter. An embryo containing this construct at the AttP2 site [48] was stained by FISH and immunostaining with antisense lacZ probe and α–Eve antibody [62] respectively. The embryo was imaged in late nuclear cycle 14 with a 20x objective on a Zeiss 710 confocal microscope. A: Eve (magenta) and lacZ (green) B: Eve in grayscale C: lacZ in grayscale.