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. 2017 Jul 17;12(7):e0181085. doi: 10.1371/journal.pone.0181085

Fig 8. Increasing substrate stiffness promotes early FAK autophosphorylation and ERK 1/2 activation.

Fig 8

(A) Representative western blots for phospho-FAK (Y397, P-FAK), total FAK, phospho-ERK 1/2 (T202/Y204, P-ERK), total ERK 1/2, and GAPDH for RPTECS (left) and NHLFs (right) 24 h after plating. (B) Densitometry for relative P-FAK per total FAK for RPTEC (left, N = 5) and NHLF experiments (right, N = 3). (C) Densitometry for relative P-ERK/Total ERK for RPTEC (left, N = 3) and NHLF experiments (right, N = 3). *: p < 0.05 relative to glass and #: p < 0.05 relative to 20 kPa and glass for multiple comparisons by ANOVA with Tukey’s post-hoc test. ANOVA with post-hoc trend analyses for P-FAK/Total FAK and P-ERK/Total ERK were highly significant (p = 0.0003 and p = 0.002, respectively, for trend from 4 Pa to Glass).