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. 2017 Jun 30;13(6):e1005585. doi: 10.1371/journal.pcbi.1005585

Fig 5. H3K79 methylation plays a causal role in repressing gene expression noise in yeast.

Fig 5

A, The absence of H3K79 methylation in DOT1 deletion strains was confirmed with western blot. B, Yeast two-color system for measuring intrinsic noise and on TEF1. C, The fluorescence intensities of GFP and dTomato among single cells were measured by FACS. Fluorescence levels are in arbitrary units. D, DOT1 deletion flattens the noise-protein level regression (regression coefficients: -0.19 in the HO deletion background vs. -0.13 in the DOT1 deletion background, linear regression, P = 0.03). E, Burst frequencies of TEF1 and SSB1 are reduced in the DOT1 deletion strain. ***, P < 0.005; **, P < 0.01.