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MOTU numbers at different minimum abundance thresholds of sequence reads: Comparisons of mock community analysis using (A) Illumina MiSeq and (B) Roche 454, and analyses of field-collected samples using (C) Illumina MiSeq and (D) Roche 454. A 0.05% of total reads for a single sample is indicated by dashed lines. Effects of the abundance threshold on MOTU numbers were evaluated both at a 97 and 99% similarity threshold for MOTU clustering.
