Table 1. PCR primers used in this study.
Primer name | Platforms | Sequence |
---|---|---|
LSU Cop-D2F [21] | MiSeq & Roche 454 | 5´-AGACCGATAGCAAACAAGTAC-3´ |
LSU Cop-D2R [21] | 5´-GTCCGTGTTTCAAGACGG-3´ | |
2nd primer for LSU Cop-D2F | MiSeq | 5´-ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGACCGATAGCAAACAAGTAC-3´ |
2nd primer for LSU Cop-D2R | 5´-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTCCGTGTTTCAAGACGG-3´ | |
3rd forward primer | MiSeq | 5´-AATGATACGGCGACCACCGAGATCTACACXXXXXXXXACACTCTTTCCCTACACGACGC-3´ |
3rd reverse primer | 5′-CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTGACTGGAGTTCAGACGTGTG-3′ |