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. 2017 Jun 30;13(6):e1006863. doi: 10.1371/journal.pgen.1006863

Fig 5. Three modes of regulation of mRNA synthesis by upstream transcripts.

Fig 5

Each panel shows the log2 RNA level in the 46a strain (“WT”, in black) and the log2 fold change in transcript level (blue) in each of the six mutant strains. Annotated protein-coding regions (ORFs) and mapped poly(A) 3’ ends [44] on the Watson (w) and Crick (c) strands are shown in red. Approximate transcript 5’ (S) and 3’ (E) ends are indicated with dotted lines. A) The HRP1 gene has a constitutive start site followed by a Sen1-dependent terminator (attenuator) that is responsive to the activity of Hrp1 and several other factors. B) The IMD2 gene has an upstream start site (S1) that is used when the cellular GTP level is high, resulting in premature termination at the attenuator to create a CUT. Functional mRNA is produced from the downstream start site (S2) when the GTP level is low. C) The SER3 gene is regulated by an independent upstream transcript, Srg1.