| 10×PCR buffer | 5 μl |
| MgCl2 (25 mmol/L) | 10 μl |
| dNTP mixture (10 mmol/L each) RNase | 5 μl |
| Inhibiter (40 U/μl) | 1 μl |
| AMV RTase XL (5 U/μl) | 1 μl |
| AMV-Optimized Taq (5 U/μl) | 1 μl |
| ADM upstream primer (20 μmol/L) | 1 μl |
| ADM downstream primer (20 μmol/L) | 1 μl |
| Total RNA samples | 1 μl |
| RNase free H2O | 4 μl |
|
| |
| Total | 30μl |
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