Table 3. Liver damage associated with catalase knockout mice (shortened).
Male wild-type (WT) and catalase knockout (Cat-/-) mice were sacrificed at >12mo of age. The liver was harvested, weighed, formalin fixed and then embedded in paraffin blocks and prepared for histological analysis using hematoxylin and eosin staining. Tissue damage was analyzed using the Brunt scoring method adapted for mice [41, 42]. Data represent the proportion of mice in each group meeting the pathological criterion.
| Pathology | # mice showing pathology | |
|---|---|---|
| WT | Cat-/- | |
| Macrosteatosis a | ||
| <10% | 6/7 | 3/6 |
| 10-33% | 1/7 | 3/6 |
| >33% | 0/6 | 1/6 |
| Microsteatosis b | ||
| any | 1/7 | 5/6 |
| Inflammation c | ||
| foci of inflammatory cells | 0/7 | 3/6 |
| lipogranulomas | 0/7 | 3/6 |
| foamy macrophages | 0/7 | 4/6 |
| Liver cell injury d | ||
| ballooning degeneration | 0/7 | 2/6 |
| Fibrosis e | 0/7 | 0/6 |
Vesicles larger than hepatocyte nucleus.
Hepatocyte filled with small vesicles, central nucleus.
Looked for lobular inflammation, foci of inflammatory cells, lipogranulomas, portal inflammation, foamy macrophages.
Looked for ballooning, acidophil bodies, pigmented macrophages, megamitochondria.
Determined by Picrosirius Red staining, imaging with polarized light in a tiling fashion across the whole tissue and analysis of the images by the 3I program Slidebook (3I, Denver, Colorado).