Table 4. Differentially-expressed liver transcripts in catalase knockout mice.
Genes involved in metabolic dysfunction were identified through a literature search. Genome-wide gene transcription profiling was obtained by RNA-sequencing of hepatic RNA from 2 mo old mice (n=3). Genes with false discovery rate (FDR)-controlled P < 0.05 and fold-change (FC) >2 or < 0.5 in Cat-/- samples relative to WT samples were arbitrarily defined as being differentially-expressed.
| Category | Gene | Gene symbol | Log2FC | FC | FDR |
|---|---|---|---|---|---|
| Obesity | Leptin receptor | Lepr | -1.431 | 0.37 | 4.62E-08 |
| Free fatty acid metabolism | Beta2 adrenoceptors | Adrb2 | -1.289 | 0.41 | 2.28E-03 |
| alpha1A adrenoceptors | Adra1a | 1.657 | 3.2 | 6.36E-11 | |
| Lipases | Lipg | 1.251 | 2.4 | 1.25E-05 | |
| Lpl | -1.749 | 0.30 | 3.88E-15 | ||
| Insulin sensitivity | Peroxisome proliferator-activated receptor gamma (PPARγ) | Pparg | 2.273 | 4.8 | 1.66E-15 |
| Insulin receptor substrate | Irs 2 | -1.804 | 0.29 | 7.81E-15 | |
| Irs 3 | 1.633 | 3.1 | 2.90E-02 | ||
| Lipid metabolism | Fatty acid translocase | Cd36 | 2.279 | 4.9 | 1.48E-18 |
| Apolipoprotein A 4 | Apoa4 | 1.614 | 3.1 | 1.42E-09 | |
| Biosynthesis | 3-Hydroxy-3-Methylglutaryl-CoA Reductase | Hmgcr | -1.122 | 0.46 | 1.77E-04 |
| Fatty Acid Synthase | Fasn | 1.423 | 2.7 | 3.71E-12 | |
| Stearoyl-CoA desaturase-1 | Scd1 | 1.332 | 2.5 | 3.75E-10 | |
| Stearoyl-CoA desaturase-2 | Scd2 | 1.213 | 2.3 | 5.52E-07 | |
| Stearoyl-CoA desaturase-3 | Scd3 | 1.104 | 2.1 | 5.88E-07 | |
| Stearoyl-CoA desaturase-4 | Scd4 | 1.254 | 2.4 | 4.38E-09 | |
| Growth and differentiation | Ribosomal Protein S6 Kinase | Rps6ka1 | 1.023 | 2.0 | 3.32E-06 |
| Glucose transport | Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (glut4) | Slc2a4 | 2.128 | 4.4 | 2.40E-07 |