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. Author manuscript; available in PMC: 2018 Mar 20.
Published in final edited form as: Chem Res Toxicol. 2017 Mar 1;30(3):785–793. doi: 10.1021/acs.chemrestox.6b00315

Figure 2. Acetylation inhibits ALDH2 activity in vitro.

Figure 2

(A) A dose dependent increase in acetylated recombinant ALDH2 coincides with increasing concentrations of acetic anhydride when a non-reducing, non-denaturing native gel is immunoblotted using a pan-acetyllysine antibody. Increasing concentrations of acetic anhydride also caused an increased migration of the acetylated protein through the native gel. (B) Recombinant ALDH2 activity subsequent to treatment with acetic anhydride as measured by the absorbance of NAD(H) at 340 nm. Significance is compared against untreated (0 mM acetic anhydride) enzyme (* p<0.05, ** p<0.01). Error bars depict the standard deviation (n=4).

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