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(A) Absorbance of NAD(H) at 340 nm is measured to assess ALDH2 activity. SIRT3 was added to acetylated ALDH2 and was able to partially recover ALDH2 activity. The lines plot the average NAD(H) produced in the triplicate wells. (B) The bar graph depicts the calculated activity of ALDH2 over 30 minutes for the activity assay (n = 3, standard deviation). (** p<0.01)
