Table 1. Kinetic parameters of recombinant ALDH2 following in vitro acetic anhydride treatment.
The decrease in kcat along with an increase in Km concurrent with increasing acetylation suggests mixed inhibition. Values listed are the average ± standard deviation.
[Acetic Anhydride] | kcat (min−1) | Km (μM) |
---|---|---|
IC0 | 15.8 ± 1.2 | 213 ± 54 |
IC25 | 14.1 ± 1.2 | 285 ± 75 |
IC50 | 12.2 ± 1.0 | 715 ± 129 |
IC75 | 11.9 ± 1.3 | 1530 ± 267 |