Table 2. SIRT3 Target Affinity Ratio (STAR) values for acetylated lysines of ALDH2.
Lysines were identified using stable isotope dilution mass spectrometry and STAR values were calculated from the integrals of the corresponding chromatograms (n ≥ 3). Numbering is for the full 517 amino acid long human protein. (A higher STAR value denotes relatively greater SIRT3 deacetylation activity towards that residue.)
| Amino Acid of ALDH2 | STAR |
|---|---|
| Lys52 | 25.5 |
| Lys73 | 13.0 |
| Lys78 | 50.1 |
| Lys81 | 100.0 |
| Lys155 | 0.9 |
| Lys159 | 3.6 |
| Lys280 | 9.8 |
| Lys355 | 18.4 |
| Lys368 | 25.2 |
| Lys369 | 26.5 |
| Lys378 | 8.1 |
| Lys383 | 9.7 |
| Lys428 | 0.0 |
| Lys451 | 7.0 |
| Lys506 | 0.3 |
| Lys511 | 5.7 |