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. Author manuscript; available in PMC: 2018 Jul 15.
Published in final edited form as: J Immunol. 2017 Jun 14;199(2):643–655. doi: 10.4049/jimmunol.1601715

Figure 2. Inhibition of T cell restricted Notch signaling reduces pulmonary leukocyte recruitment to the lungs during cryptococcal infection.

Figure 2

(A) Total cell numbers were determined from hemocytometer counts of digested suspensions from perfused lungs at 3, 4 and 6 wpi. Differential cell counts were calculated from Wright-Giemsa stained cytospin slides at (B) 3, (C) 4 wpi and (D) 6 wpi. Data shown represent the mean and standard error from 2-4 pooled experiments with a total n=7-21 mice per time point. (E-F) RNA was isolated from naïve and infected lung leukocytes from perfused mice at 4 wpi. Expression of (E) MIP-1β (Ccl4) and (F) MIP-2α (Cxcl2) in lung leukocytes were measured by qRT-PCR and normalized to Gapdh. Data shown are the mean ± SEM of n=3-7/group. *p<0.05, **p<0.01