Figure 3. Inhibition of Notch signaling does not significantly alter accumulation or frequency antigen-primed of T cells in the lungs during cryptococcal infection.

(A) Lung leukocytes were isolated from perfused CCD and WT mice at 4 wpi and the total number of TCRβ+ T cells and number of CD4⁺ T cells were quantified after gating on Live, CD45⁺ cells by flow cytometry. (B) CD44 and CD62L staining was used to assess activation and effector phenotype of CD4⁺ T cells and representative plots from 1 of 2 experiments are shown. Data shown in quadrants are the mean frequency of CD4⁺ parent ± SEM with n=5-6/group. None of data were significantly different.