FIG 1 .

Zika virus infection of primary hBMECs. (A) Primary hBMECs were infected with ZIKV (PRVABC59) at an MOI of 10, and 12 to 72 hpi ZIKV antigen-positive cells were detected by anti-DENV4 HMAF. (B to D) Titers of ZIKV-infected hBMEC supernatants were determined in an FFU assay (B) and analyzed for cellular ZIKV RNA levels by qRT-PCR (C) and for infected cells (D). (E to G) hBMECs and Vero E6 cells were pretreated with IFN-α (1,000 U/ml) for 3 h prior to ZIKV infection (MOI, 10) (E), or IFN-α was added at the indicated time postinfection and infected Vero E6 (F) or hBMECs (G) were immunostained and quantitated 24 later.