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hBMECs are viable after ZIKV infection. (A) Vero E6 or hBMECs were infected with ZIKV (MOI, 10), and costained 3 dpi with calcein-AM (green [live cells])/propidum iodide (red [dead cells]). Following calcein-AM/PI staining, monolayers were fixed and immunostained for ZIKV antigen. (B) Viability of ZIKV-infected Vero E6 cells and hBMECs was assessed via CyQuant NF uptake 3 dpi, and results were compared to those for mock-infected controls.
