FIG 4 .

ZIKV-infected hBMECs are viable and productive following cellular passage. (A and B) ZIKV-infected Vero E6 cells or hBMECs (MOI, 10) were trypsinized and passaged (1:3) 3 dpi and every 3 days thereafter. ZIKV-infected passaged hBMECs were detected by immunostaining. (C) Cells were infected and passaged as for panels A and B, and cell viability was assessed via calcein-AM/PI staining and fluorescent image overlay. (D) ZIKV titers in supernatants of hBMECs consecutively passaged 1 to 3 times (every 3 days).