FIG 3 .

var2csa intronless mutant produces VAR2CSA PfEMP1 protein. (A) Western blot analysis of 30-hpi iRBC membrane extracts performed using antibodies against VAR2CSA PfEMP1 (top left panel) or the PfEMP1 ATS region (top right panel). Extracts were prepared from a CSA-panned FCR3 culture, intron KO clone A, and a 3D7 WT bulk culture. An amido black staining of the Western blot is shown as a loading control. Molecular masses are indicated at the left. (B) Immunofluorescence analysis of live 30-hpi iRBCs containing CSA-panned FCR3, 3D7 WT bulk, intron KO clone A, or intron KO telomere mutant clone parasites. DNA was stained with DAPI (magenta), and iRBCs were surface labeled with specific antibodies against VAR2CSA PfEMP1 (green). Fluorescent images are shown in the top panel, and wide-field merged images are shown in the bottom panel. Scale bar, 10 μm.