TABLE 2 .
Polyphosphate quantification in M. tuberculosis wild-type and Pst/SenX3-RegX3 mutant strainsa
| Strain | nmol polyP/mg total protein (mean ± SD)b |
P value (versus WT) |
|---|---|---|
| WT | 0.09 ± 0.02 | |
| ΔphoY1 | 0.13 ± 0.06 | 0.258 |
| ΔphoY2 | 0.41 ± 0.17 | 0.010 |
| ΔphoY2/pMVphoY2 | 0.19 ± 0.06 | 0.023 |
| ΔphoY1 ΔphoY2 | 0.86 ± 0.21 | 0.0003 |
| ΔphoY1 ΔphoY2/pMVphoY1 | 0.08 ± 0.02 | 0.413 |
| ΔphoY1 ΔphoY2/pMVphoY2 | 0.07 ± 0.02 | 0.205 |
| ΔphoY1 ΔphoY2 ΔregX3 | 0.64 ± 0.18 | 0.001 |
| ΔphoY1 ΔphoY2 ΔregX3/pNDregX3 | 0.44 ± 0.10 | 0.0003 |
| ΔpstA1 | 0.56 ± 0.15 | 0.001 |
| ΔpstA1/pMVpstA1 | 0.21 ± 0.10 | 0.051 |
| ΔregX3 | 0.07 ± 0.02 | 0.306 |
| ΔpstA1 ΔregX3 | 0.09 ± 0.05 | 0.957 |
| ΔpstA1 ΔregX3/pNDregX3 | 0.45 ± 0.22 | 0.016 |
a
Strains were grown in 20 ml of 7H9 broth until mid-exponential growth phase (OD600 of 0.5) and pelleted by centrifugation prior to polyphosphate extraction.
b
Results are mean values ± standard deviations of at least four independent experiments.