Figure 2. Kin-10 knockdown increased lipofuscin accumulation and Nile red staining in C. elegans.

A. Knockdown of kin-10 increased lipofuscin accumulation. Representative autofluorescence images of worms at day 1 or 8 of adulthood (upper panel). The fluorescence intensity (bottom panel) was quantified using the ImageJ software by determining the average pixel intensity (n > 80 per condition). B. Representative Nile red staining images (upper panel) of worms at day 1 or 8 of adulthood and relative fluorescence intensity (bottom panel) are shown. The fluorescence intensity was quantified using ImageJ software by determining the average pixel intensity (n = 20 per condition). Values indicate mean ± SEM. Bars that do not share a common letter (a, b, c) are significantly different among the groups at P < 0.05. All experiments in this figure were carried out at 21 °C.