Figure 4. Effect of kin-10 knockdown on the lifespan of age-1, akt-1, and daf-16 single mutant worms.

Age-1(hx546) A., akt-1(mg144) B., and daf-16(mu86) C. single mutant worms were fed on the control (L4440) or kin-10 RNAi plates under standard conditions. n = 60 per condition (A and B); n = 40 per condition (C). Viability was scored as movement away from pick touch at the indicated days. Kin-10 knockdown successfully suppressed the longevity extension by age-1(hx546) and akt-1(mg144) mutations, but did not synergistically shorten the lifespan of daf-16(mu86) mutant. All experiments in this figure were carried out at 21 °C.