Table 1. Epitope mapping: mouse 12G4 vs human 3C23K.
Position AA | 12G4 binding (AU fluorescence) | 3C23K binding (AU fluorescence) | ||
---|---|---|---|---|
Peptide 1 | 1–20 | PPNRRTCVFFEAPGVRGSTK | < 1000 | < 1000 |
Peptide 2 | 11–30 | EAPGVRGSTKTLGELLDTGT | < 1000 | < 1000 |
Peptide 3 | 21–40 | TLGELLDTGTELPRAIRCLY | < 1000 | < 1000 |
Peptide 4 | 31–50 | ELPRAIRCLYSRCCFGIWNL | < 1000 | < 1000 |
Peptide 5 | 41–60 | SRCCFGIWNLTQDRAQVEMQ | 37300 ± 5000 | 28400 ± 5000 |
Peptide 6 | 51–70 | TQDRAQVEMQGCRDSDEPGC | 24500 ± 4300 | 26000 ± 5100 |
Peptide 7 | 61–80 | GCRDSDEPGCESLHCDPSPR | < 1000 | < 1000 |
Peptide 8 | 71–90 | ESLHCDPSPRAHPSPGSTLF | < 1000 | < 1000 |
Peptide 9 | 81–100 | AHPSPGSTLFTCSCGTDFCN | < 1000 | < 1000 |
Peptide 10 | 91–110 | TCSCGTDFCNANYSHLPPPG | < 1000 | < 1000 |
Peptide 11 | 101–120 | ANYSHLPPPGSPGTPGSQGP | < 1000 | < 1000 |
Peptide 12 | 111–130 | SPGTPGSQGPQAAPGESIWM | < 1000 | < 1000 |
Peptide 13 | 113–132 | GTPGSQGPQAAPGESIWMAL | < 1000 | < 1000 |
Control | 47 | WNLTQDRAQVEMQGCRDSDE | 11200 ± 2000 | 16600 ± 2300 |