Table 2. Binding of 3C23K to 13 human sequences homologous to the 7AA epitope sequence DRAQVEM identified by BLAST.
| Protein* | Peptide sequence** | 3C23K binding (AU fluorescence) |
|---|---|---|
| MISRII (Q16671) | WNLTQDRAQVEMQGCRDSDE | 5200 ± 600 |
| kinesin-like protein KIFC3 (AAH47051/Q9BVG8) | IEEAFARAQVEMKAVHENLA | 310 ± 80 |
| BRLZ, brain leucine zipper protein (AAR83719/A6NC98) | ARLELERAQLEMQSQQLRES | 1100 ± 100 |
| unnamed protein product (BAG62992/B4DWD0) | NQLKDAIAQVEMDLKRLRDP | < 200 |
| integrin alpha D, CD11d (AAB60630/Q13349) | GQEAFMRAQMEMVLEEDEVY | < 200 |
| tektin 2 (EAX07391/Q9UIF3) | SRFNKDRAEAEMKAATELRE | < 200 |
| DNA polymerase iota chain A (3H40_A/Q9UNA4) | VDLDCFYAQVEMISNPELKD | < 200 |
| Nup205 (nucleoporin) (AAH44255/Q92621) | QLDFFDRAQIEQVIANCEHK | 520 ± 20 |
| integrin alpha D precursor (NP_005344/Q59H14) | GQEAFMRAQMEMVLEEDEVY | < 200 |
| titin, isoform N2-B (NP_003310/Q8WZ42) | EGILTDRAQIEVTSSFTMLV | < 200 |
| PITPNB (phosphatidylinositol transfer protein) (CAQ68294/B3KYB6) | HIDIADRSQVEPADYKADED | < 200 |
| LYPDC1 (AAH40046/Q8IXM0) | QERVDDRAEVEKRLREGEED | < 200 |
| TMEM64 (transmembrane protein 64) (AAI13829/Q6YI46) | MFYVVHRAQVELNAAIVACE | < 200 |
| PMS2 (mismatch repair endonuclease) (BAD89425/P54278) | PSDPTDRAEVEKDSGHGSTS | < 200 |
* Genbank and Uniprot references are indicated in parenthesis.
** Residues known to be critical for 12G4 binding (25) are in bold and identical residues found in the same position in the DRAQVEM sequence of MISR-II are underlined.