Table 3. Affinity constants (K_D) of low-fucosylated 3C23K and glycosylated 3C23K-HEK293 for the different human Fcγ receptors.

	Antibody
Receptor	3C23K	3C23K-HEK293
Fc©RI/CD64	0.1–2.7*	0.2–3.6*
Fc©RIIa/CD32a	176	182
Fc©RIIbc/CD32bc	316	378
Fc©RIIIa/CD16a	1–12*	31–164*
Fc©RIIIb/CD16b	86	378

Affinity constants are expressed as K_D in nM. *K_D1 and K_D2 were calculated by using heterogeneous fitting model.

The K_D values, expressed in nM, were calculated by using a steady-state fitting model, but for FcγRI and FcγRIIIa. FcγRI and FcγRIIIa do not adhere to the one-to-one binding model and fit well using a heterogeneous ligand model (60). The fitting curves and data are given in supplementary data. The K_D values of 3C23K and 3C23K-HEK293 were obtained with the same fitting model, thus allowing comparing the data for each receptor, although the precise binding mechanism has not been elucidated yet and the affinity values reported can vary (60).