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. 2017 Jun 6;2(3):e00015-17. doi: 10.1128/mSystems.00015-17

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Copyright © 2017 Burger et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — Comparing TSAS- and iRsp1140-predicted essential genes. We used TSAS to identify genes essential for aerobic and photosynthetic growth in Sistrom’s minimal medium and compared these to iRsp1140 essential gene predictions (aerobic [A] and photosynthetic [B]). We then updated iRsp1140 based on inspection of the genes that were predicted to be essential by either Tn-seq or TSAS alone, resulting in iRsp1140_opt. These changes to the model resulted in increased agreement with TSAS-predicted essential genes for aerobic (C) and photosynthetic (D) growth.