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. 2017 Jul 5;174(15):2512–2527. doi: 10.1111/bph.13862

Figure 8.

Figure 8

Dioscin reverses the effects of Sirt1 siRNA. (A) Effects of dioscin on the viability of NRK‐52E and HK‐2 cells with or without transfection of Sirt1 siRNA in vitro. (B) Effects of dioscin on the protein levels of Sirt1, Keap1, Nrf2 and acetylated NF‐κB after the transfection of Sirt1 siRNA in NRK‐52E and HK‐2 cells. (C) Effects of dioscin on the mRNA levels of IL‐1β, IL‐6 and TNF‐α after transfection of Sirt1 siRNA in NRK‐52E and HK‐2 cells. (D) Effects of dioscin on the expression of Sirt1 in NRK‐52E and HK‐2 cells with or without transfection of Sirt1 siRNA based on immunofluorescence staining in vitro (200× magnification). Data are presented as the mean ± SD (n = 5). # P < 0.05, significantly different from CDDP groups; *P < 0.05, significantly different from CDDP group transfected with Sirt1 siRNA.