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. 2017 Jun 21;174(15):2457–2470. doi: 10.1111/bph.13855

Figure 2.

Figure 2

Bouchardatine activates the AMPK signalling pathway via LKB1 in 3T3‐L1 adipocytes. Confluent 3T3‐L1 pre‐adipocytes were exposed to an adipogenic cocktail (IBMX, dexamethasone, and insulin) for 9 days in the presence or absence of Bou. (A) Dose‐dependent effect of Bou on the LKB1–AMPK pathway. *P < 0.05 compared with undifferentiated group; #P < 0.05 compared with DMSO group. (B) Time‐dependent effect of Bou on the LKB1–AMPK pathway. *P < 0.05 compared with DMSO group. (C) Effect of Bou in the presence or absence of NAM on the expression of the LKB1–AMPK pathway. *P < 0.05 compared with DMSO group; #P < 0.05 compared with Res or Bou group. (D) Effect of LKB1 on the TG levels. LKB1 plasmid transfection as described in the Methods section. Original magnification, 100×. Scale bar, 100 μm. * P < 0.05 compared with DMSO group; #P < 0.05 compared with Bou or Res group. (E) Effect of LKB1 transfection on the AMPK pathway. *P < 0.05 compared with DMSO group. Data were quantified from six independent experiments.