Figure 7.
Binding sites of μ‐TRTX‐Df1a over hNaV.7. Chimeras hNaV1.7/rKV2.1 containing the paddles S3‐S4 from DI‐DIV from NaV1.7 were used to explore the binding site of Df1a over NaV1.7. Potassium currents were elicited by depolarization to +70 mV. The currents are shown before and after addition of 1 μM Df1a toxin. sDf1a (both C‐terminally acid and amide) preferentially binds to S3‐S4 loop region in DII of NaV1.7, followed by DIII and DIV. Df1a (C‐terminally acid and amide) had no effect on wild‐type rKV2.1 at up to 1 μM. Data are from n = 5 independent experiments for each condition assayed; one oocyte was considered for each independent experiment.