Table 1.
Primer sequences of ABCA2, ABCA3 and GAPDH
| Gene | Primer sequence (5′ to 3′) | Primer length (base pairs) | Amplicon length (base pairs) | Tm | GC% |
|---|---|---|---|---|---|
| ABCA2 | F:CCGCACCATCCTTCTGTCCACCCACC | 26 | 263 | 70.79 | 65.38 |
| R:TGCGGATGAACTGGGACACCTGGAGC | 26 | 70.31 | 61.54 | ||
| ABCA3 | F:GGCCATCATCATCACCTCCCACAGCA | 26 | 177 | 66.7 | 57.7 |
| R:AGCGCCTCCTGTTGCCCTTCACTCTG | 26 | 68.7 | 61.5 | ||
| GAPDH | F:GCCCCAGCAAGAGCACAAGAGGAAGA | 26 | 106 | 68.64 | 57.69 |
| R:CATGGCAACTGTGAGGAGGGGAGATT | 26 | 66.38 | 53.85 |
Abbreviations: F, forward; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; R, reverse; Tm, melting temperature.