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. 2017 Jul 18;8:1333. doi: 10.3389/fmicb.2017.01333

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Copyright © 2017 Domenech and García.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescent labeling of the non-encapsulated S. pneumoniae strain R6 with HPA. Exponentially growing cultures of S. pneumoniae R6 in C+Y medium were incubated with the indicated concentrations of the lectin and observed for fluorescence (HCX PL FLUOTAR 40×/0.75 objective). Merges of fluorescence and phase-contrast images are also shown; bar = 25 μm. Enlarged view of two diplococci showing reduced fluorescence at the equatorial zone of growth (indicated by arrows; 63× objective). Bar = 2 μm.