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Lack of HPA labeling in ethanolamine-containing streptococci. S. pneumoniae R6 cells were incubated for several generations in Cden medium containing ethanolamine (Cden-EA) (A,D). A portion of the culture then received choline chloride (5 μg/ml) and incubation proceeded at 37°C for 3 h (B,E). The S. mitis SK598 strain was grown in THY medium (C,F). The three cultures were incubated with HPA (25 μg/ml) (D–F). Bar = 25 μm.
