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. 2017 Jul 18;8:1293. doi: 10.3389/fmicb.2017.01293

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Copyright © 2017 Padilla, Zulian, Ferreres, Pastor, Esteve-Zarzoso, Beltran and Mas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Yeast species population dynamics established by RFLP-ITS-PCR of LYS-cultured isolates. (A) Mixed fermentation (four species of non-Saccharomyces and the three strains of Saccharomyces), (B) Fermentation performed using the three native strains of Saccharomyces. SM, Synthetic must; GR, Grenache must; CA, Carignan must. The species identified were Hanseniaspora guilliermondii , Metschnikowia pulcherrima , Torulaspora delbrueckii , Starmerella bacillaris , Hanseniaspora uvarum , Saccharomyces cerevisiae . Columns are indicated as: 24 h (24 h after non-Saccharomyces inoculation), 24 h Sc (24 h after S. cerevisiae inoculation), M (middle fermentation), F (end of fermentation).

Inline graphic — Yeast species population dynamics established by RFLP-ITS-PCR of LYS-cultured isolates. (A) Mixed fermentation (four species of non-Saccharomyces and the three strains of Saccharomyces), (B) Fermentation performed using the three native strains of Saccharomyces. SM, Synthetic must; GR, Grenache must; CA, Carignan must. The species identified were Hanseniaspora guilliermondii , Metschnikowia pulcherrima , Torulaspora delbrueckii , Starmerella bacillaris , Hanseniaspora uvarum , Saccharomyces cerevisiae . Columns are indicated as: 24 h (24 h after non-Saccharomyces inoculation), 24 h Sc (24 h after S. cerevisiae inoculation), M (middle fermentation), F (end of fermentation).