Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 18;8:1245. doi: 10.3389/fmicb.2017.01245

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Tian, Li, Huang, Tian, Liu, Yang, Liu, Yan, Xu, Li and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Purification and Western blot analysis of recombinant proteins of EaGAPDH and EmGAPDH with anti-Eimeria chicken sera. Lane M: standard protein molecular weight marker; (A) Purification of EaGAPDH and EmGAPDH; lane 1: Inclusion body extracted from pET32a-EaGAPDH transformed E. coli; lane 2: EaGAPDH purified through Ni₂+-charged column chromatography; lane 3: Inclusion body extracted from pET32a-EmGAPDH transformed E. coli; lane 4: EmGAPDH purified through Ni₂+-charged column chromatography; (B) Western blot analysis of recombinant proteins of EaGAPDH and EmGAPDH; lane1: EaGAPDH recognized by anti-E. acervulina chicken serum; lanes 2 and 4: EaGAPDH recognized by serum of uninfected chicken; lane 3: EmGAPDH recognized by anti-E. maxima chicken serum.