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. 2017 Jul 18;8:1245. doi: 10.3389/fmicb.2017.01245

Table 2.

Primers used for the quantitative RT-PCR.

RNA target Primer sequence Accession No. Amplification efficiency (%) Correlation coefficients (r2)
IFN-γ Forward: 5′-AGCTGACGGTGGACCTATTATT-3′ Y07922 99.16 0.9976
Reverse: 5′-GGCTTTGCGCTGGATTC-3′
IL-2 Forward: 5′-TCTGGGACCACTGTATGCTCT-3′ AF000631 98.53 0.9932
Reverse: 5′-ACACCAGTGGGAAACAGTATCA-3′
TNFSF15 Forward: 5′-CCTGAGTTATTCCAGCAACGCA-3′ NM_001024578 98.51 0.9992
Reverse: 5′-ATCCACCAGCTTGATGTCACTAAC-3′
IL-17D Forward: 5′-GCTGCCTCATGGGGATCTTTGGTG-3′ EF570583 98.18 0.9954
Reverse: 5′-CGATGACGGCTTGTTCTGGTTGAC-3′
TGF-β4 Forward: 5′-CGGGACGGATGAGAAGAAC-3′ M31160 97.39 0.9981
Reverse: 5′-CGGCCCACGTAGTAAATGAT-3′
IL-4 Forward: 5′-ACCCAGGGCATCCAGAAG-3′ AJ621735 99.41 0.9996
Reverse: 5′-CAGTGCCGGCAAGAAGTT-3′
GAPDH Forward: 5′-GGTGGTGCTAAGCGTGTTAT-3′ K01458 95.48 0.9994
Reverse: 5′-ACCTCTGTCATCTCTCCACA-3′

Amplification efficiency (%) = (10-1/slope–1) × 100.