Table 2.
RNA target | Primer sequence | Accession No. | Amplification efficiency (%)∗ | Correlation coefficients (r2) |
---|---|---|---|---|
IFN-γ | Forward: 5′-AGCTGACGGTGGACCTATTATT-3′ | Y07922 | 99.16 | 0.9976 |
Reverse: 5′-GGCTTTGCGCTGGATTC-3′ | ||||
IL-2 | Forward: 5′-TCTGGGACCACTGTATGCTCT-3′ | AF000631 | 98.53 | 0.9932 |
Reverse: 5′-ACACCAGTGGGAAACAGTATCA-3′ | ||||
TNFSF15 | Forward: 5′-CCTGAGTTATTCCAGCAACGCA-3′ | NM_001024578 | 98.51 | 0.9992 |
Reverse: 5′-ATCCACCAGCTTGATGTCACTAAC-3′ | ||||
IL-17D | Forward: 5′-GCTGCCTCATGGGGATCTTTGGTG-3′ | EF570583 | 98.18 | 0.9954 |
Reverse: 5′-CGATGACGGCTTGTTCTGGTTGAC-3′ | ||||
TGF-β4 | Forward: 5′-CGGGACGGATGAGAAGAAC-3′ | M31160 | 97.39 | 0.9981 |
Reverse: 5′-CGGCCCACGTAGTAAATGAT-3′ | ||||
IL-4 | Forward: 5′-ACCCAGGGCATCCAGAAG-3′ | AJ621735 | 99.41 | 0.9996 |
Reverse: 5′-CAGTGCCGGCAAGAAGTT-3′ | ||||
GAPDH | Forward: 5′-GGTGGTGCTAAGCGTGTTAT-3′ | K01458 | 95.48 | 0.9994 |
Reverse: 5′-ACCTCTGTCATCTCTCCACA-3′ |
∗Amplification efficiency (%) = (10-1/slope–1) × 100.