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. 2017 Jul 18;8:1245. doi: 10.3389/fpls.2017.01245

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Copyright © 2017 Qi, Wang, Sun, Day, Guo and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological observations of necrotic cell death in wheat leaves treated with BSMV and infected with incompatibility race CYR23. (A) Necrotic cell death was observed by epifluorescence at 24, 48, and 120 hpi. HMC, haustorial mother cell; IH, infection hypha; HR, hypersensitive response; SV, sub-stomatal vesicle. Bar, 20 μm. (B) Necrotic cell death was quantified as the area of auto-fluorescence. (C) Hyphal lengths were quantified from the junction of the sub-stomatal vesicle to the hyphal tip. (D) Colony size was measured as the area of auto-fluorescence. Asterisks indicate a significant differences (P < 0.05) from BSMV:γ using the Student’s t-test.