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. 2017 Jul 17;7:5525. doi: 10.1038/s41598-017-05876-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vammin efficiently induces blood perfusion and edema in skeletal muscles. (A) Images with Doppler and CPS ultrasound from the semimembranosus muscles 6 days after the gene transfer. Increased perfusion is seen in all treated muscles. Edema is observed with both doses (B) Quantification of the blood perfusion from CPS ultrasound (n = 4–6 per group) (C) Quantification of edema from the ultrasound images (n = 4–6 per group). In B to C the data is presented as mean ± SEM. * P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.