Table 2.
Kinetic parameters of the interaction of RTA with human stalk complexes analyzed by bio-layer Interferometry.
| Protein complex | *K D (M−1) | *k on (M−1 s−1) | *k off (s−1) |
|---|---|---|---|
| P1-P2 | (2.18 ± 0.24) × 10−8 | (1.75 ± 0.39) × 105 | (3.71 ± 0.45) × 10−3 |
| P1-P2ΔC | (9.03 ± 0.17) × 10−8 | (3.54 ± 0.04) × 104 | (3.19 ± 0.04) × 10−3 |
| P1ΔC-P2 | N/A | N/A | N/A |
| P1ΔC-P2ΔC | N/A | N/A | N/A |
| P2-P2 | (2.19 ± 0.97) × 10−6 | (1.72 ± 0.09) × 104 | (3.85 ± 1.87) × 10−2 |
| P2ΔC-P2ΔC | N/A | N/A | N/A |
| ΔuL10(P1-P2)2 | (4.07 ± 1.99) × 10−9 | (4.19 ± 1.98) × 105 | (1.31 ± 0.03) × 10−3 |
| ΔuL10(P1-P2ΔC)2 | (5.32 ± 0.03) × 10−9 | (2.29 ± 0.01) × 105 | (1.22 ± 0.05) × 10−3 |
| ΔuL10(P1ΔC-P2)2 | (1.12 ± 0.02) × 10−8 | (1.94 ± 0.27) × 105 | (2.16 ± 0.26) × 10−3 |
*K D, k on and k off values represent the average of three replicate experiments.