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. 2017 Jul 17;7:5517. doi: 10.1038/s41598-017-04811-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation the expression pattern of prognostic genes in HCC samples. (A) Western blot analysis of UPB1 (upper panel), SOCS2 (middle panel), and RTN3 (lower panel) in 7 paired normal and tumor tissue samples. (B) Western blot quantitative densitometry of the UPB1, SOCS2, and RTN3 relative expressions. Proteins was quantified and normalized to β-actin. Statistical analysis was done using Wilcoxon rank test. Data are expressed as mean values ± SD. *p < 0.05, **p < 0.01. (C) Representative images from immunohistochemistry (IHC) staining of UPB1 (upper panel), SOCS2 (middle panel), and RTN3 (lower panel) in 82 normal tissues and paired HCC tissues. Left panel: 100 × magnifications; right panel: 200 × magnifications. Bars = 100 μm. (D) Statistical analyses of the average IHC scores of UPB1 (upper panel), SOCS2 (middle panel), and RTN3 (lower panel). Statistical analysis was done using Wilcoxon rank test. Data are expressed as mean values ± SD.