Table 1.

Sequences of oligonucleotide primers used in this study.

Forward primer, 5′–3′	Reverse primer, 5′–3′
Cloning into pPICZα A
Efe-afpA-2
GTATCTCTCGAGAAAAGAGCGGAGACTGGCATTCTGA	AAGCTGGCGGGCCGCCTAATGACACGTGACAGCTC
Efe-afpA-3
GTATCTCTCGAGAAAAGAATCACGTATGAAGGAACATGTT	AAGCTGGCGGGCCGCCTAATGACACGTGACAGCTC
Deletion of propeptide and Kex2 sites of the α-factor signal sequence
Efe-afpA-3
ATCACGTATGAAGGAACATGTTC	AGCTAATGCGGAGGATGC
Sequencing recombinant plasmids
5′ AOX1 primer
GACTGGTTCCAATTGACAAGC