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. 2017 Jul 17;7:5519. doi: 10.1038/s41598-017-05736-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The rescue effect of p38 inhibitor (SB202190) on F2075C-mutated cells obtained by ENU mutagenesis screening or reconstructed F2075C-mutated cells. (A,B) Effect of SB202190 on wild-type CD74-ROS1, ENU-screened F2075C-mutated cells (A), or reconstructed F2075C cells with or without 1 μg/mL DOX (B). Each cell line was treated with the indicated dose ranges of inhibitors for 72 h, then cell viability was measured by the CellTiter-Glo assay. Each value is shown as relative cell growth by normalizing with the values of untreated wells. (C,D) Immunoblotting of SB202190-treated wild-type CD74-ROS1 cells or F2075C-mutated cell (C), or SB202190- or cabozantinib-treated reconstructed F2075C-mutated cells with or without 1 μg/mL DOX (the cells shown DOX (+) were pretreated with DOX for 24 h.) (D). Cell lysates were immunoblotted to detect the indicated proteins. Uncropped blots are presented in Supplementary Fig. S11.